Alicia Oshlack Murdoch Childrens Research Institute The Royal Children’s Hospital Location: Carslaw 173 Time: 2pm Friday, September 23, 2011 Dealing with the data deluge: differential expression analysis for RNA-seq Abstract: New high-throughput DNA sequencing technologies have been rapidly developing over the past 5 years and are routinely able to produce millions of short DNA reads. These technologies are being widely used to study the transcriptome in which steady state RNA is sequenced (RNA-seq). These technologies produce vast amounts of data which need to be analysed in order to make biological inference. RNA-seq data are complex and the analysis involves a series of steps for which research is ongoing. In this talk I will describe the data and outline the major steps involved in analysing an RNA-seq dataset, focusing on methods to determine differentially expressed genes between samples and to perform gene set testing.